• 经测试应用WB, Flow Cytmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

• 阳性对照
  • Jurkat, HepG2, HeLa and K562 cell lysates; permeabilized HeLa cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at -20ºC.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• 纯度Tissue culture supernatant
• 克隆单克隆
• 克隆编号EPR8779
• 同种型IgG
• 研究领域
  • Signal Transduction
   
  • Metabolism
   
  • Amino Acids

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Amino acid metabolism

应用

• 应用说明Is unsuitable for ICC,IHC-P or IP.

靶标

• 功能Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells.
• 组织特异性Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.
• 疾病相关Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme.
• 序列相似性Belongs to the HAM1 NTPase family.
• 细胞定位Cytoplasm.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 3704 Human
  • Entrez Gene: 16434 Mouse
  • Entrez Gene: 311422 Rat
  • Omim: 147520 Human
  • SwissProt: Q9BY32 Human
  • SwissProt: Q9D892 Mouse
  • SwissProt: D3ZW55 Rat
  • Unigene: 415299 Human

  see all
• 别名
  • C20orf37 antibody
  • dJ794I6.3 antibody
  • HLC14-06-P antibody

  see all

Anti-Inosine triphosphate pyrophosphatase antibody [EPR8779] 图像

• 

  Western blot - Anti- Inosine triphosphate pyrophosphatase antibody [EPR8779] (ab150420)
  All lanes : Anti-Inosine triphosphate pyrophosphatase antibody [EPR8779] (ab150420) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : K562 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 21 kDa
  
• 

  Flow Cytometry - Anti- Inosine triphosphate pyrophosphatase antibody [EPR8779] (ab150420)
  Flow cytometric analysis of permeabilized HeLa cells labeling Inosine triphosphate pyrophosphatase with ab150420 at 1/10 dilution(red) comapred with a rabbit IgG negative control (green).