Zeocin is supplied a sterile filtered blue solution at 100 mg/ml in HEPES buffer.Zeocin is a selective antibiotic that acts on both eukaryotic and prokaryotic cells. Resistance to Zeocin® is conferred by the Sh ble gene from Streptoalloteichus hindustanus1-3. Zeocin® is the commercial name for a special formulation containing Phleomycin, a copper-chelated glycopeptide antibiotic isolated from a mutant strain of Streptomyces verticillus. This antibiotic of the bleomycin family exhibits activity against bacteria, eukaryotic microorganisms, plant and animal cells. Although bleomycin antibiotics perturb plasma membranes, their activity is generally believed to be related to their ability to bind and intercalate DNA thus destroying the integrity of the double elix.